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. 2014 Nov 25;6(4):e982949. doi: 10.4161/19382014.2014.982949

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© 2014 The Author(s). © 2014 Published with license by Taylor & Francis Group, LLC

© Matthew Riopel, Jinming Li, George F Fellows, Cynthia G Goodyer, and Rennian Wang

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 3. — Glycogen expression in the developing human fetal pancreas. (A) Transmission electron micrographs of the 8 week human fetal pancreas showed glycogen positivity in ductal cells. Scale bars = 1 μm. L = lumen, Mit = mitochondria, N = nuclei, RER = rough endoplasmic reticulum. (B) PAS stain (pink) indicates glycogen, which is highly expressed in ducts between 12–15 weeks. By 20 weeks of fetal age, glycogen expression is reduced in ductal cells and is expressed primarily in surrounding acinar cell clusters with no islet or ductal cell expression in the adult pancreas. Highlighted areas are enlarged in B₁. Scale bar = 50 μm. Representative PAS images from at least 3 pancreata per age group are shown. A = acinar; CA = centroacinar; IC = endocrine cell cluster; L = lumen of duct; asterisks = blood vessels.