Table 1. In vitro sensitivity of 3D7, 3D7L272F, FCB and FCBC101F (in presence or absence of verapamil, VP) to antimalarial drugs.
| Drug† | Mean ± SEM IC50 values for individual parasite strains/lines‡ |
|||||
|---|---|---|---|---|---|---|
| 3D7 | 3D7L272F | FCB | FCBC101F | FCB + VP | FCBC101F + VP | |
| CQ | 15 ± 1.8 | 6.1 ± 1.5* | 187 ± 7.1 | 34 ± 1.8* | 47 ± 1.2 | 14 ± 1.0 |
| QN | 176 ± 17 | 108 ± 13* | 333 ± 5.8 | 220 ± 23* | 161 ± 33 | 223 ± 31 |
| QD | — | — | 167 ± 13 | 62 ± 3.1* | 46 ± 1.9 | 46 ± 2.9 |
| MQ | 87 ± 29 | 64 ± 17 | 13 ± 1.1 | 14 ± 0.9 | 8.8 ± 1.0 | 18 ± 2.0 |
| AQ | 25 ± 2.3 | 27 ± 2.1 | — | — | — | — |
| MDAQ | 14 ± 1.5 | 15 ± 1.5 | 52 ± 4.1 | 18 ± 1.0* | 16 ± 1.0 | 9.1 ± 0.7 |
| PPQ | 18 ± 4.1 | 22 ± 5.3 | 12 ± 0.6 | 27 ± 1.8* | 13 ± 0.4 | 25 ± 3.1 |
| ART | 3.5 ± 1.2 | 4.1 ± 1.6 | 13 ± 0.9 | 9.1 ± 0.7** | 9.9 ± 0.9 | 9.1 ± 0.5 |
| BSD (μM) | 1.5 ± 0.6 | 47 ± 6.0* | — | — | — | — |
| AMT (μM) | — | — | 5.6 ± 0.6 | 465 ± 54* | 11 ± 0.5 | 687 ± 32 |
†CQ, chloroquine; QN, quinine; QD, quinidine; MQ, mefloquine; AQ, amodiaquine; MDAQ, monodesethyl amodiaquine; PPQ, piperaquine; ART, artemisinin; BSD, blasticidin; AMT, amantadine; VP, verapamil (used at 0.8 μM).
‡IC50 values are listed in nM, except where indicated, and are show as the mean ± SEM. n = 3 independent assays (each performed as a single replicate for FCB parasites and in quintuplicate for 3D7 parasites). Significantly different mean IC50 values relative to controls (F-test; *p < 0.05, **p < 0.01).