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. 2015 Sep 30;5:14664. doi: 10.1038/srep14664

Figure 4. Cryo-immunogold EM micrographs of PROSPR isolated EVs.

Figure 4

Presence of tetraspanin CD9 (10 nm colloidal gold particles) was observed as attached to the internal/external face of the membrane in single layered exosome-like vesicles (a,b) and in multilayered exosome-like vesicles (c). Presence of the ESCRT protein Alix was also confirmed (10 nm colloidal gold particles) in exosome-like vesicles isolated by PROSPR from blood plasma (df). Scale bars in all images represent 40 nm.