Fig. 3.

Expression of MAPKs and p-MAPKs in NOC15-treated Jurkat T cells. (a) Western blot. (b) Relative expression. The cells were preincubated for 22 h and then stimulated with PMA plus ION for 2 h. After the cells were treated by NOC15 (0. 0.25, 0.5, 1, 2, and 4 μmol/l) for 24 h, the cells were collected, lysed, and the proteins were extracted for western blot analysis. The β-actin was used as the internal control. The results are expressed as means±SD for three independent experiments. *P<0.05 versus untreated control. The expressions of p-p38 and p-ERK1/2 were significantly increased in a dose-dependent manner. ERK1/2, phospho-extracellular signal-regulated protein kinase 1/2; ION, ionomycin; MAPK, mitogen-activated protein kinase; NOC15, N-farnesyloxy-norcantharimide; PMA, phorbol 12-myristate 13-acetate.