FIG 1.

Functional complementation of trans-splicing mutant F19. (a) Test for photoautotrophic growth of wild-type strain 137C, trans-splicing mutant strain F19, and selected transformants (T404-2/6, T401-1/1, T401-2/1, and T401-2/2) obtained by transformation of F19 with BAC 31O13. Cells were spread onto acetate-containing (TAP) or acetate-lacking (HS) agar plates at the indicated cell densities and incubated under high-light conditions (HL). 137C and complemented strains were able to grow on HS or Tris-acetate-phosphate plates under high light, in contrast to mutant F19, which is photosynthetically deficient. (b) Northern analysis of 137C, F19, and complemented transformant strains. Filters were consecutively hybridized with probes specific for psaA exon 1, tscA, and RBSC1 (as a loading control) transcripts. (c) Immunodetection of the PsaA protein in 137C, F19, and four transformants. A Coomassie-stained gel served as a loading control.