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. 2015 Sep 30;8:498. doi: 10.1186/s13071-015-1108-7

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© Yin et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Construction of DNA and adenovirus vaccines encoding ubiquitin-conjugated multistage antigen segments derived from T. gondii. a Schematic representation of the compound proteins, which encode amino acid sequences of eight protein segments derived from tachyzoite, bradyzoite and sporozoite antigens of T. gondii. b Construction of recombinant DNA and adenovirus vaccines expressing ubiquitin-conjugated multistage antigen segments. The recombinant eukaryotic plasmids pVAX1-MAS (c), pVAX1-UMAS (d), and pHBAd-MCMV-GFP-UMSA (e) were identified by restriction enzyme digestion analysis (Lane 1). Lane M: DNA marker. f Recombinant adenoviral particles were obtained from 293 cells