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. 2015 Sep 30;8:498. doi: 10.1186/s13071-015-1108-7

Fig. 3.

Fig. 3

The assay of subtype of T cells by FCM from immunized mice. Four weeks after the last immunization splenocytes from mice were collected and stained with anti-CD8a-PE monoclonal antibodies and anti-CD4-FITC antibodies. Percentage of positive stained cells were analyzed by FCM (a, b). *indicates statistically significant differences between the marked group