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Deletion mutant ranking depends on cell survival over time under conditions of trimtheprim exposure. Stationary phase cultures of W3110 and single-gene mutants were diluted 100-fold and exposed to 64 μg/ml trimethoprim for 3, 5, and 7 days. The data for each mutant was plotted and compared with that of W3110. Error bars indicate the standard deviation (n = 3). The asterisk indicates statistical significance as determined using Mann–Whitney U tests (∗P < 0.05).
