Fig. 1.

Schematic representation of the different APP split-luciferase constructs. (A) Schematic representation of the different human APP and APP C-terminal fragments generated for fusion to the humanized Gaussia luciferase moieties (hGLuc). APPΔC corresponds to APP695 deleted from its intracellular C-terminal domain (stop after the KKKQY intracellular sequence). C99 and C83 correspond to the APP β and α C-terminal fragments, respectively. All the N-terminally truncated CTFs are fused to the APP signal peptide (SP). Abbreviations are as follows: TM, transmembrane; JM, juxtamembrane; AICD, APP intracellular domain; ext, extracellular; int, intracellular. The positions of Flemish and mutant 5 (mut5) mutations are underlined and amino acid substitutions are in red. The cleavage sites of α (α)-, β (β)- and γ (γ and ε)-secretases are indicated by arrows. (B) Schematic representation of APP constructs fused to hGLuc moieties (hGLuc1 and hGLuc2). The epitopes of the human-specific W0-2 antibody, the APP C-terminal and hGLuc antibodies are indicated.