Fig. 4.

Comparison of APP and APP C-terminal fragment dimerization. Cells were transfected with the control empty vector (mock), the APP-hGLuc1 and 2, C99-hGLuc1 and 2 or C83-hGLuc1 and 2 constructs. (A) Protein expression was monitored in cell lysates by Western blotting with the W0-2, Cter or hGLuc antibodies. (B) Luciferase activity was measured and expressed as RLU normalized to APP (set to 100%). Values (means ± SEM) are representative of 3 independent experiments (n = 4 in each experiment). ^***p < 0.001, n.s. (non-significant), as compared to APP-hGLuc1 and 2.