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. 2015 Sep 6;5:763–773. doi: 10.1016/j.fob.2015.09.002

Fig. 5.

Fig. 5

Effects of gamma-secretase inhibition on APP C99 and C83 dimerization. CHO cells transfected with the empty vector (mock) or the luciferase constructs expressing either APP, C99 and C83 or APP-hGLuc1 and 2, C99-hGLuc1 and 2 and C83-hGLuc1 and 2. Cells were treated with DAPT 1 μM for 18 h. (A) Expression of the non-tagged proteins and effect of the treatment on metabolism were checked in cell lysates by western blotting with APP specific antibodies (W0-2 and Cter). (B) Expression of the hGLuc fusion proteins was checked in cell lysates by Western blotting with the hGLuc antibody and APP specific antibodies (W0-2 and Cter). (C) Luciferase activity measured was expressed as RLU normalized to APP-hGLuc1 and 2 (set to 100%). Values (means ± SEM) are representative of 3 independent experiments (n = 4 in each experiment). *p < 0.05, ***p < 0.001, n.s. (non-significant), as compared to APP-hGLuc1 and 2. (D) Gamma-secretase inhibition was confirmed by monitoring Aβ38, Aβ40 and Aβ42 production by ECLIA in the culture medium of cells expressing APP-hGLuc1 and 2 or C99-hGLuc1 and 2. Results are given as Aβ levels in pg/ml. Values (means ± SEM) are representative of 3 independent experiments (n = 4 in each experiment). *p < 0.05 and ***p < 0.001, as compared to non-treated cells.