Fig. 7.

Influence of the intracellular in APP dimerization. (A) Protein expression was monitored in cell lysates by Western blotting with the W0-2 or hGluc antibodies (left panel). Luciferase activity was measured and expressed as RLU normalized to APP (set to 100%, right panel). Values (means ± SEM) are representative of 2 independent experiment (n = 4 in each experiment). ^***p < 0.001, as compared to APP-hGLuc1 and 2. (B) sAPPα and β production of APP-hGLuc1 and 2 and APPΔC-hGLuc1 and 2 constructs were monitored by ECLIA in the culture media of cells and are given in ng/ml. ^*p < 0.05 and ^***p < 0.001, as compared to non-transfected cells or as indicated. Ratio of sAPPα on sAPPβ produced was calculated in the same experiments. ^**p < 0.01, as compared to APP-hGLuc1 and 2 (C) Immunostaining of cells co-expressing APP or APPΔC constructs of either. Nuclei were stained with DAPI, and APP fusion constructs were stained by the W0-2 and/or hGLuc antibodies. Scale bar: 5 μm.