Fig. 2.

Role of TRIM25 in MAVS-mediated signaling. (A) TRIM25 is required for efficient activation of IFN-β promoter by MAVS. Wild-type (WT) and TRIM25 knock-out (TRIM25 KO) MEFs were transfected with vector or MAVS plasmids (hMAVS). Cells were co-transfected with IFN-β promoter-reporter and TK-Renilla reporter plasmids. Promoter activities were determined by Dual-Luciferase assay 16 h after transfection. (B) TRIM25 is required for efficient activation of NF-κB promoter by MAVS. WT and TRIM25 KO MEFs were transfected with vector or MAVS plasmids together with NF-κB reporter and TK-Renilla reporter plasmids. Promoter activities were determined using procedures similar to those in (A). (C) Enhanced MAVS-induced IFN-β promoter activity by TRIM25 overexpression. HEK293T cells were transfected with vector, MAVS and TRIM25 plasmids as indicated, together with IFN-β reporter and TK-Renilla reporter plasmids. Promoter activities were determined using procedures similar to those in (A). (D) Enhanced MAVS-induced NF-κB promoter activity by TRIM25 overexpression. HEK293T cells were transfected with expression plasmids and NF-κB reporter plasmids as indicated. Promoter activities were determined using procedures similar to those in (C). Results of experiments, performed in triplicate, are presented as means ± standard deviation.