Fig. 1.
Disintegration of the chromosomal subdomains by BAF53 knockdown. HeLa cells stably expressing GFP-tagged histone H2B were transfected with siRNA-BAF53. (A) The expression levels of H2B-GFP and BAF53 were determined by immunoblotting. (B) The expression level of H2B-GFP of a cell was measured by integrating the signals from all of the pixels of the midsection image of a fixed nucleus revealing GFP-tagged histone H2B (t-test; P > 0.5). Error bars, mean ± SD (n = 10). (C) Representative images of the midsection of a fixed nucleus revealing GFP-tagged histone H2B. Pixels with gray values above the assigned thresholds (TH = 40 and TH = 60) are highlighted in red. (D) Distributions of the occupancies of red pixels in the control and BAF53-knockdown cell nuclei. Boxes represent median and quartile values, and bars define the 5th and 95th percentiles (n = 30). t-test; *P < 0.001.