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The dissociation of DNA loops from the nuclear scaffold in BAF53 knockdown cells. (A) Nonfixed nuclei on coverslips were submitted to the maximum fluorescent halo technique (MFHT) for DNA loop-size measurements. Immunostaining with an anti-lamin antibody delimitated the matrix from DNA loops. Scale bar, 5 μm. (B) The mean of the halo length of each cell was plotted versus the standard deviation of the halo length of the cell. t-test; P < 0.001.
