Figure 1.

ISL1 is expressed in Rathke's pouch during development. ISL1 immunostaining (green) and DAPI counterstaining (blue) was carried out in sections from developing pituitary glands collected from e11.5 to P3. The photographs of sagittal e11.5, e12.5, and e13.5 sections were taken at ×200, and the later ages, sagittal e14.5, e16.5, and coronal P3, at ×100. Scale bars, 100 μm. White box inset of P3 photo was taken at ×630. RP, Rathke's pouch; VD, ventral diencephalon; AL, anterior lobe; IL, intermediate lobe; PL, posterior lobe; VH, ventral hypothalamus.