Figure 2.
Sirt1 activation decreases osteoclastogenesis via a FoxO-dependent mechanism. Representative pictures (A) and number (B) of TRAP-positive multinucleated osteoclasts derived from BMMs of mice of the indicated genotypes cultured with M-CSF (30 ng/mL) and RANKL (30 ng/mL) in the presence or absence of Sirt1 activators (5μM SRT2104, 10μM SRT3025) for 4 days. C, Western blotting of BMMs, which were pretreated with vehicle (DMSO) or SRT3025 (10μM) for 30 minutes, followed by M-CSF (30 ng/mL) for 8 hours. D, Macrophage cell number in cultures derived from mice of the indicated genotypes in the presence of M-CSF (30 ng/mL) for 2 days. Bars represent mean ± SD; #, P < .05 vs untreated cells within the same genotype; *, P < .05 vs untreated cells from FoxO1,3,4f/f by two-way ANOVA.