Figure 3.

Changes in the hepatic lipidome of HFD mice treated with Ad-PLIN5. Lipids extracted from the livers of 6 weeks HFD mice treated with Ad-GFP or Ad-PLIN5 and harvested at fed ad libitum in late morning as in Materials and Methods were analyzed by Q-Exactive LC-MS/MS as in Materials and Methods. A, The representative total ion chromatogram of lipids in Ad-GFP liver in full MS. B–D, Relative intensity of neutral lipids (B), phospholipids (C), and Cers (D) determined as in Materials and Methods was compared between liver from Ad-GFP and Ad-PLIN5 mice. PG, phosphatidylglycerol; PS, phosphatidylserine; PI, phosphatidylinositol. Data are mean ± SEM; n = 3; *, P < .05; **, P < .01; n.s., not significant.