Figure 4.

Profiles of the TG lipid class in the livers of HFD mice overexpressing PLIN5. A, Relative abundance (peak area) of the 100 most abundant TG species identified in the liver of Ad-GFP (white)- and Ad-PLIN5 (black)-treated mice (6-week HFD). TGs are grouped based on their total number of carbons (c) and unsaturation (number of double bonds). Data are mean ± SEM; n = 3; *, P < .05. B, Correlation between the unsaturation of C14 to C18 fatty acyl chains expressed as the number of double bonds/total carbon and abundance of fatty acyl chains expressed as an area ratio in Ad-PLIN5 over Ad-GFP liver of the TG species as appeared in A. C, Quantitative PCR determined the expression of genes involved in liver injury in livers of Ad-PLIN5 male mice fed HFD (6-week HFD). Levels are presented taking the levels of Ad-GFP on the corresponding duration of HFD as 100%. Expression levels were normalized using β-actin as an internal control. Data are mean ± SEM; n = 5–6; *, P < .05; **, P < .01. Afp, α-fetoprotein (AFP); Col1a2, collagen α1(II chain); H19, imprinted maternally expressed transcript (nonprotein coding).