Figure 4.

Genetic inactivation of Klf11 leads to increased energy expenditure. Klf11+/+ and KLF11−/− female mice were subjected to CLAMS measurements to monitor their energetic status after 13 weeks on a HFD. In metabolic cages, food intake (A) was measured, and food intake/body weight (B) was calculated. Over a 48-hour period, including day and night of 24 hours fed and 24 hours fasted, measurements were made for total activity (C), ambulation (D), rearing (E), heat (F), metabolic rate (G), VO₂ (H), VCO₂ (I), and respiratory coefficient (J). Additional measurements in CLAMS included resting (K) and active (L) energy expenditure to calculate the total energy expenditure (M). Each group consisted of 8 mice (*, P < .05, t test).