Table 1.
Antibody Table
| Antibody | Peptide/Protein Target | Antigen Sequence (if known) | Name of Antibody | Manufacturer, Catalog Number, and/or Name of Individual Providing the Antibody | Species Raised in; Monoclonal or Polyclonal | Dilution Used |
|---|---|---|---|---|---|---|
| Primary | ATGL | ATGL (30A4) rabbit mAb | Cell Signaling, 2439 | Rabbit; monoclonal | 1:100 | |
| Secondary | N/A | Alexa Fluor 488 donkey antirabbit IgG (H + L) antibody | Life Technologies, A-21206 | Donkey; polyclonal | 1:200 | |
| Primary | F4/80 | Anti-F4/80 antibody [CI: A3-1] | Abcam, ab6640 | Rat; monoclonal | 1:100 | |
| Secondary | N/A | Antirat IgG (whole molecule)-TRITC antibody | Sigma-Aldrich, T4280 | Rabbit; polyclonal | 1:200 | |
| Primary | CD11c | Hamster antimouse CD11c | BD Pharmingen, 550283 | Hamster; monoclonal | 1:100 | |
| Secondary | N/A | Alexa Fluor 488 goat antirat IgG (H + L) antibody | Life Technologies, A-11006 | Goat; polyclonal | 1:500 | |
| Primary | CD11c | Antimouse/human Mac-2 (Galectin-3), purified (clone M3/38) (rat IgG2a) | Cedarlane Laboratory, CL8942AP | Rat; monoclonal | 1:100 | |
| Secondary | N/A | Antirat IgG (H + L) | Jackson ImmunoResearch, 112-035-003 | Goat; polyclonal | 1:500 | |
| Secondary | N/A | Cy3-AffiniPure goat anti-Armenian hamster IgG (H + L) | Jackson ImmunoResearch, 127-165-160 | Goat; polyclonal | 1:1000 | |
| Primary | Akttotal | NP_001014421 | Anti-Akt1/PkBα antibody | EMD Millipore, 07-416 | Rabbit; polyclonal | 1:250 |
| Primary | AktpSer473 | Antiphospho-Akt1/PKBα (Ser473) antibody | EMD Millipore, 05-736 | Rabbit; monoclonal | 1:250 | |
| Primary | AktpThr308 | Antiphospho-Akt1/PKBα (Thr308) antibody | Cell Signaling, 9275S | Rabbit; polyclonal | 1:250 | |
| Primary | RAN GTPase | Human RAN, aa 7-171 | Ran | BD Biosciences, 610340 | Rat; monoclonal (clone 20/Ran) | 1:10 000 |
| Primary | CD16/CD32 | Antimouse CD16/CD32 | eBioscience, 14-0161-86 | Rat; monoclonal (clone 93) | N/A | |
| Primary | CD45 | Anti-CD45-PerCP | BD Biosciences, 557235 | Rat; monoclonal (clone 30-F11) | N/A | |
| Primary | CD11b | Anti-CD11b-PeCy7 | eBioscience, 552850 | Rat; monoclonal (clone M1/70) | N/A | |
| Primary | CD11c | Anti-CD11c-APC | eBioscience, 550261 | Rat; monoclonal (clone HL3) | N/A | |
| Primary | F4/80 | Anti-F4/80-Alexa Fluor 780 | eBioscience, 474801 | Rat; monoclonal (clone BM8) | N/A |