Table 1.
The top five CpG sites with the largest effect size and FDR-adjusted P-value < 0.05 for associations between maternal pre-pregnancy obesity or underweight and offspring cord blood methylation
| Exposure | Gene region (CpG) | CHR | Gene feature group | Relation to CpG Island | β (95% CI)* | FDR-adjusted P-value |
|---|---|---|---|---|---|---|
| Pre-pregnancy maternal obesity | unnamed (cg00526953) | 16 | North shore | −14.99 (−20.89, −9.1) | 0.016 | |
| SUCLG2 (cg02321096) | 3 | 3'UTR | −11.4 (−15.78, −7.02) | 0.012 | ||
| FAM129B (cg03270036) | 9 | Gene body | North shore | −9.65 (−12.92, −6.37) | 0.003 | |
| KIF15;KIAA1143 (cg17546649) | 3 | TSS1500; Gene body | North shore | 7.6 (4.61, 10.59) | 0.016 | |
| STAB2 (cg23131355) | 12 | TSS1500 | −5.05 (−6.98, −3.12) | 0.011 | ||
| Pre-pregnancy maternal underweight | TEX14 (cg01796478) | 17 | 5'UTR | Island | 17.57 (8.86, 26.28) | 0.021 |
| ZNF783 (cg02373627) | 7 | Gene body | Island | −17.19 (−24.4, −9.98) | 0.003 | |
| MVD (cg27467516) | 16 | Gene body | Island | −16.55 (−24.94, −8.16) | 0.027 | |
| TMEM201 (cg07232095) | 1 | Gene body; 3'UTR | −13.44 (−18.8, −8.08) | 0.002 | ||
| CFHR5 (cg13682187) | 1 | TSS200 | 11.88 (6.83, 16.94) | 0.004 |
Models were adjusted for bisulfite conversion batch, offspring sex, maternal age, smoking status, occupation and parity.
CHR, chromosome; TSS-1500, 1500 base-pairs from a transcription start site; TSS-200, 200 base-pairs from a transcription start site; UTR, untranslated region. Shores are defined according to NCBI guidelines and labelled north (upstream) or south (downstream) of islands.
*β coefficient is mean difference in percentage methylation compared with offspring of normal weight mothers