Fig. 2.

Multiple alignments of the BrAOP2, BoAOP2, and AtAOP2 protein sequences. Multiple alignments were performed using MEGA v.6.0. AtAOP2 is a known functional protein from Arabidopsis thaliana (ecotype Cvi), and BoAOP2 is from B. oleracea (collard). The solid lines above the alignment indicate the consensus sequences for the DIOX-N and 2OG-FeII_Oxy domains identified using the Conserved Domain Database (Marchler-Bauer et al., 2011)). The black shading indicates that at least four proteins share the same amino acid site. Asterisks show four active-site residues in the 2OG-FeII_Oxy domain.