Fig. 3.

Evolutionary relationships and motif structures of Brassicaceae AOP proteins. Phylogenetic analysis was performed based on the conserved sequences at the N and C terminal parts of AOP proteins using the neighbour-joining method (bootstrap test with 1000 replicates) (Tamura et al., 2011). The sequenced Brassicaceae species in BRAD included: lineage I species Arabidopsis thaliana, Arabidopsis lyrata, Capsella rubella, Camelina sativa, and L. alabamica; lineage II species T. halophila, T. salsuginea, S. parvula (synonym of T. parvula), S. irio, B. rapa, B. oleracea, and R. sativus; and an early branching sister of the core Brassicaceae, Aethionema arabicum. Black branches indicate AOP1 proteins, coloured branches indicate AOP2 and AOP3 proteins. The tree is drawn to scale; bar, number of substitutions per site.