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. 2015 Jul 16;66(20):6399–6413. doi: 10.1093/jxb/erv348

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© The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2. — 2D gel separation of seed proteins and the effect of ageing on seed protein abundance. (A) 2D gel of total soluble proteins from dry seeds stained with silver nitrate. The areas indicated on the gel (1 and 2) are enlarged in panels B and C. (B) Area 1 selected on the gel depicting the abundance of protein spot ID1345 that contains RPS12C and TPX1 proteins for the four genotypes (Ler and the near-isogenic lines NILGAAS1, NILGAAS2 and NILGAAS5) at two physiological states [after-ripened (AR) and aged]. (C) Area 2 showing the change in abundance of protein spot ID0667, corresponding to VTE1 protein, for the four genotypes at two physiological states. Arrows indicate the position of the proteins.