Table 1.
Genotype specific protein spots that were identified in (A) both physiological state and genotype comparisons and (B) in either physiological state or genotype comparison for near-isogenic lines NILGAAS1, NILGAAS2 and NILGAAS5 (Fig. 4C−E)
The table displays protein spots based on the seven comparisons. Spot ID, the gene corresponding to the protein underlying the spot, molecular weight (MW in kD) and the theoretical (Th) and experimental (Exp) isoelectric point (pI), are presented respectively. Furthermore the relative abundance (fold change) of the spots in both types of comparisons (Fig. 1; physiological state and genotype) is indicated. Positive fold changes indicate higher abundances, and negative lower abundances. Spots in bold exhibit seed longevity up or seed longevity down protein profile. Fold changes in bold indicate statistically significant changes. NG1, NILGAAS1; NG2, NILGAAS2; NG5, NILGAAS5. Spots that were identified based on comparison to the reference protein map (http://www.seed-proteome.com) are labelled with R. n.i., not identified.
| A | Spot ID | Gene | Protein | MW (kDa) | pI | Relative abundance (fold change) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Th | Exp | Th | Exp | Physiological state: Aged vs. AR | Genotype AR: NIL vs. Ler | |||||||||
| Ler | NG1 | NG2 | NG5 | NG1 | NG2 | NG5 | ||||||||
| NILGAAS1 | ||||||||||||||
| ID1279 | n.i. | n.i. | n.i. | 22.82 | n.i. | 8.68 | -1.0 | 2.5 | -1.1 | 1.5 | -2.6 | 1.3 | -1.3 | |
| eID0255 R | AT1G03890 | Cupin family protein | 49.67 | 29.33 | 5.45 | 5.63 | -1.0 | 1.9 | 1.0 | 1.0 | -2.3 | -1.0 | -1.0 | |
| ID0426 | n.i. | n.i. | n.i. | 65.51 | n.i. | 5.41 | 1.2 | 1.8 | 1.2 | 1.4 | -1.7 | -1.4 | -1.2 | |
| ID1104 | AT1G03880 | Cruciferin B | 50.56 | 27.38 | 7.0 | 5.61 | 1.1 | 1.5 | 1.0 | 1.1 | 1.9 | 1.1 | 1.0 | |
| AT5F35590 | 20S proteasome alpha subunit | 27.29 | 5.66 | |||||||||||
| AT1G03890 | Cupin family protein | 49.67 | 5.45 | |||||||||||
| eID0138 R | AT4G28520 | Cruciferin C | 58.24 | 25.40 | 6.99 | 5.81 | 1.9 | 1.5 | 1.5 | 1.6 | 2.3 | 1.3 | 1.0 | |
| ID0994 | AT1G54870 | Oxidoreductase family protein | 36.76 | 32.75 | 8.76 | 5.65 | 2.3 | 1.7 | 2.0 | 2.4 | 1.5 | 1.2 | 1.3 | |
| AT4G28520 | Cruciferin C | 58.24 | 6.99 | |||||||||||
| ID0715 | AT1G03880 | Cruciferin B | 50.56 | 47.74 | 7.00 | 6.02 | -1.2 | -1.6 | -1.3 | -1.6 | 1.6 | -1.0 | 1.1 | |
| AT1G74960 | Fatty acid biosynthesis 1 | 57.60 | 7.93 | |||||||||||
| ID0537 | AT2G14170 | Aldehyde dehydrogenase 6B2 | 53.40 | 58.77 | 5.79 | 5.68 | -2.0 | -3.1 | -2.3 | -2.0 | 1.5 | 1.3 | 1.1 | |
| AT5G08670 | ATP synthase beta chain 1 | 59.63 | 6.53 | |||||||||||
| AT5G08680 | ATP synthase beta chain | 59.86 | 6.45 | |||||||||||
| AT5G08690 | ATP synthase beta chain 2 | 59.71 | 6.60 | |||||||||||
| NILGAAS2 | ||||||||||||||
| ID0458 | AT3G20050 | T-complex protein 1 alpha subunit | 59.23 | 63.81 | 6.22 | 5.87 | -1.2 | 1.1 | 1.5 | 1.1 | -1.4 | -2.0 | -1.2 | |
| ID0955 | n.i. | n.i. | n.i. | 38.44 | n.i. | 7.29 | 1.4 | -1.7 | -2.1 | -1.8 | 1.4 | 1.7 | 1.5 | |
| NILGAAS5 | ||||||||||||||
| eID0228 | AT5G19510 | Elongation factor EF1B | 24.20 | 43.24 | 4.17 | 3.88 | -1.6 | 1.3 | 1.2 | 1.7 | -1.3 | -1.1 | -1.6 | |
| ID1146 | AT2G31670 | Unknown protein | 28.86 | 25.36 | 6.96 | 5.10 | 1.1 | 1.3 | 1.4 | 1.8 | -1.0 | -1.3 | -1.5 | |
| B | Spot ID | Gene | Protein | MW (kD) | pI | Relative abundance (fold change) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Th | Exp | Th | Exp | Physiological state: Aged vs. AR | Genotype AR: NIL vs. Ler | |||||||||
| Ler | NG1 | NG2 | NG5 | NG1 | NG2 | NG5 | ||||||||
| NILGAAS1 | ||||||||||||||
| ID0765 | AT3G52880 | Monodehydroascorbate reductase 1 | 50.16 | 43.31 | 8.38 | 6.08 | -1.3 | -1.3 | -1.4 | 1.3 | 2.6 | -1.0 | -1.6 | |
| ID0712 | AT5G28840 | GDP-D-mannose 3ʹ,5ʹ-epimerase | 42.76 | 45.14 | 6.15 | 5.78 | 1.2 | 1.2 | -1.0 | -1.0 | 1.9 | -1.1 | 1.0 | |
| ID0734 | AT5G28840 | GDP-D-mannose 3ʹ,5ʹ-epimerase | 42.76 | 44.99 | 6.15 | 5.74 | 1.2 | 1.7 | 1.2 | 1.5 | 1.1 | -1.1 | -1.1 | |
| ID0951 | AT1G18080 | Receptor for activated C kinase 1A | 35.75 | 38.56 | 7.81 | 7.13 | -1.1 | -1.6 | -1.3 | -1.8 | 1.6 | 1.4 | 1.4 | |
| NILGAAS2 | ||||||||||||||
| ID0206 | AT5G52300 | Responsive to dehydration 29B | 65.97 | 94.21 | 4.81 | 5.05 | -1.5 | -1.2 | -1.7 | -1.5 | -1.3 | -1.2 | 1.1 | |
| ID0196 R | AT5G52300 | Responsive to dehydration 29B | 65.97 | 94.21 | 4.81 | 5.00 | -1.4 | -1.9 | 1.0 | 1.1 | 1.2 | -1.6 | -1.5 | |
| ID0762 | AT2G15430 | RNA polymerase II | 35.46 | 42.82 | 4.39 | 4.46 | -1.3 | -1.1 | -1.6 | -1.1 | -1.4 | -1.1 | -1.3 | |
| NILGAAS5 | ||||||||||||||
| ID1505 | AT4G27160 | Napin AT2S3 | 18.76 | 9.85 | 7.87 | 8.69 | 1.5 | 1.1 | -1.2 | 1.7 | -1.0 | 1.2 | 1.2 | |
| ID0632 | AT5G38470 | Radiation sensitive 23D | 40.07 | 50.00 | 4.29 | 4.60 | 1.1 | -1.1 | -1.0 | -1.1 | 1.0 | -1.1 | -2.0 | |
| ID0258 | AT3G15670 | Late embryogenesis abundant protein | 24.19 | 26.57 | 9.43 | 8.95 | -1.2 | -1.0 | 1.1 | 1.1 | 1.0 | -1.1 | -1.9 | |
| ID1144 | AT3G15670 | Late embryogenesis abundant protein | 24.19 | 29.73 | 9.43 | 7.79 | -1.1 | -1.2 | -1.1 | -1.5 | 1.0 | 1.0 | 1.2 | |
| ID0976 | AT3G17520 | Late embryogenesis abundant protein | 32.56 | 36.11 | 5.02 | 5.54 | 1.2 | 1.2 | 1.4 | 1.6 | 1.0 | -1.1 | -1.2 | |
| ID0448 | AT2G19900 | NADP-dependent malic enzyme 1 | 64.28 | n.i. | 6.73 | n.i. | -1.3 | -1.7 | -1.7 | -1.7 | 1.1 | -1.1 | -1.0 | |