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. 2015 Jul 13;66(20):6415–6429. doi: 10.1093/jxb/erv353

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© The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 5. — Expression level correlation between RNA-Seq and qPCR. Negative correlation between FPKM values of RNA-Seq and average Ct values of qPCR indicate a consistent estimation of the relative expression levels between the two methods. The graphs (A)–(H) represent the genes: MluLR17108 (psbH), MluLR17433 (psbI), MluLR14810 (psbK), MluLR17106 (ycf4), MluLR17402 (petE), MluLR5294 (OAT4), MluLR4566 (RH57), MluLR17105 (rps4), respectively. The R in the graphs indicates the correlation coefficient. ** represents the significant level (P <0.01, Spearman’s rank correlation test). Sequences of PCR primers are given in Table 4. Red and blue dots represent individuals sampled from JH and QG, respectively.