Fig. 7.

Genetic analysis of the double mutant abi4-1 rrl and the hybrid abi4-1 OE-4. (A, B) F₂ generation seedlings of crossed plants were subjected to single nucleotide polymorphism (SNP) identification to isolate abi4 mutation loci (A) and semi-quantitative RT-PCR to determine expression of RRL (B). ACTIN2 was used as the internal control. (C) Seeds were sown on MS medium supplemented with 1.0 μM ABA. Photographs were taken at day 5 after stratification. Scale bar=0.5cm. (D) Seeds were sown on MS medium supplemented with the indicated concentration of ABA. Germination rates (%) were analysed at day 5 after stratification. Values are the means ±SE of three independent experiments (n=100). (E) Seedlings grew for 10 d after transfer from MS medium to MS medium supplemented with 10 μM ABA. Seedlings were transferred from ABA-free medium to ABA-containing medium 48h after stratification. Scale bar=0.5cm. (F) Primary root lengths were assayed after seedlings were transferred to MS medium supplemented with 0, 5, 10, 20, or 40 μM ABA for 10 d. Values are the means ±SE of three independent experiments (n=50). (This figure is available in colour at JXB online.)