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. 2015 Jul 28;66(20):6447–6459. doi: 10.1093/jxb/erv358

Fig. 5.

Fig. 5.

Mannan (LM21 antibody) distribution in TW and OW after 4 weeks of inclined growth. (A) 1 µm resin section of TW side showing strong binding of LM21 in the maturing and mature G-fibres. The numbers in the squares indicate similar areas to those shown in detail in B–D. (B) 100 µm unembedded section of developing G-fibres of area 1 showing a thin layer of labelling in the SCW/G-layer (arrow). (C) 100 µm unembedded section of more mature fibres showing labelling at the SCW/G-layer interface. (D) 100 µm unembedded section of very thick-walled mature G-fibres showing binding of the LM21 antibody in the SCW and also some more diffuse labelling in the G-layer. (E) 1 µm resin section of OW side showing very weak binding to fibre cell walls. (F) Detail of fibres in (E) showing light labelling of cell walls, possibly S1 layer of the SCW. Bars: A, E, 100 µm; B, C, D, F, 10 µm.