Fig. 8.
Expression of SxCobL4and SxFLA12 genes in transverse sections probed with antisense probes. (A) COBRA gene expression after 1 week of tipping is shown by the purple staining on the TW side of the stem. (B) A 2-week tipped stem showing that SxFLA12 gene expression occurs on the TW side of the stem. (C) Lack of purple colour in a control upright stem indicates no expression of the COBRA gene after 1 weeks of growth. (D) 1-week inclined stem shows strong expression of the COBRA gene in the G-fibres of the TW side. (E, F) After 2 and 4 weeks of tipping, respectively, strong expression of COBRA gene is shown in the G-fibres. (G–I) No COBRA gene expression is detected in OW of the same stems in D, E, and F, respectively. Bars: A–C: 100 µm, D–I: 100 µm.