Table 2.
Histological analysis of anthesis ovary and fully grown fruit of fs8.1 NILs
For each genotype, six ovaries and 18 fruits from six plants were investigated. The two values per organ represent two biological replicates. The upper value was obtained from family 13S140, and lower value was obtained from the 13S117/118 families. NA, not analysed; NS, not significant.
Parameter | Ovary | Fruit | ||||
---|---|---|---|---|---|---|
fs8.1 | WT | Pvalue | fs8.1 | WT | P value | |
Ovary wall/pericarp cell number (proximal–distal direction, 1/2 fruit) | 163.33±2.67 | 135.94±1.00 | 2.28E–06 | 499.34±7.09 | 420.89±13.31 | 2.08E–04 |
169.03±1.41 | 144.89±2.74 | 1.44E–05 | ||||
Ovary wall/pericarp cell number (mediolateral direction, 1/2 fruit) | 227.67±1.27 | 220.63±0.71 | 6.93E–04 | 413.59±2.10 | 404.16±4.03 | NS |
225.58±5.01 | 226.54±2.94 | NS | ||||
Ovary wall/pericarp cell size (proximal–distal direction, 100 µm2) | 0.1145±0.0431b | 0.1150±0.0261b | NS | 1178.38±42.10a | 1275.56±31.67a | NS |
0.1030±0.0596b | 0.1039±0.0280b | NS | 864.57±23.74b | 1018.18±31.61b | 3.03E–03 | |
Ovary wall/pericarp cell size (mediolateral direction, 100 µm2) | 0.0804±0.0680b | 0.0776±0.0950b | NS | 1308.24±34.90a | 1316.85±23.87a | NS |
0.0768±0.0612b | 0.0727±0.0879b | NS | 954.57±22.83b | 1097.29±28.10b | 2.77E–03 | |
Ovary wall/pericarp cell layer | 13.22±0.21 | 11.80±0.25 | 1.15E–04 | 32.50±0.33 | 27.45±0.42 | 6.85E–07 |
13.77±0.26 | 12.37±0.08 | 4.26E–03 | ||||
Ovary wall/pericarp thickness (mm) | 0.1112±0.0012 | 0.0983±0.0011 | 1.10E–05 | 9.56±0.07 | 8.77±0.14 | 3.13E–04 |
0.1097±0.0072 | 0.0999±0.0010 | NS | ||||
Ovary columella cell number (proximal–distal direction) | 136.78±2.39 | 114.56±3.16 | 2.26E–04 | NA | NA | NA |
136.20±3.57 | 125.27±3.07 | 4.88E–02 | ||||
Columella cell size (μm2) | 125.49±5.84 | 123.56±6.75 | NS | NA | NA | NA |
117.41±11.64 | 100.11±6.10 | NS |
a Average size of six largest cells.
b Average cell size.