Fig. 1.

Undifferentiated hNSCs and predifferentiated cell phenotypes in vitro. Immunofluorescent confocal images of undifferentiated SVZ-hNSCs in culture expressed HuNu (red) and SOX2 (green) (a), and Ki67 (red) and Nestin (green) (b), with colocalization giving a yellow appearance. Undifferentiated SVZ-hNSCs immunopositive for HuNu (red) (c–f) in culture were not observed to express Tuj1 (green) (c), GABA (green) (d), GAD (green) e, or DCX (green) f. Predifferentiated cells cultured for 7 days demonstrated immunoreactivity for HuNu (red) g–m and colabeled with Tuj1 (green) (g), GABA (green) (h), GAD (green) (i), and DCX (green) (j). Predifferentiated cells HuNu (red) cultured for 7 days demonstrated little immunoreactivity for Ki67 (green) (k), Nestin (green) (l), or SOX2 (green) (m). Scale bar: (a–f) 100 μm, (g–m) 50 μm. DCX doublecortin, GABA gamma-aminobutyric acid, GAD glutamate decarboxylase 65&67, HuNu human specific nuclear antigen, Tuj1 β-III tubulin