Fig. 2.

Functional outcome following transplantation. Effects of transplantation on contralateral limb use when rearing (a) in the cylinder test after ET-1-induced stroke. Data are mean ± standard error of the mean (SEM) expressed as a percentage of contralateral (impaired) forelimb use. Each rat acted as its own control; results following stroke were compared with 0-hour prestroke scores. ϕP <0.05, ϕϕP <0.01, ϕϕϕϕP <0.0001 relative to 0-hour poststroke baseline scores for vehicle-treated rats (n = 7); **P <0.01, ***P <0.001, ****P <0.0001 compared with 0-hour poststroke baseline scores for undifferentiated treated rats (n = 7); #P <0.05, ##P <0.01 relative to 0-hour poststroke baseline scores for predifferentiated treated rats (n = 7); δP <0.05, δδP <0.01 vehicle-treated rats compared with predifferentiated treated rats; φφP <0.01 vehicle-treated rats compared with undifferentiated treated rats; †P <0.05 predifferentiated treated rats compared with undifferentiated treated rats (two-way ANOVA followed by Bonferroni post test). Combined neurological deficit scores (b). Data presented as box plots include hinges extending from the 25th to 75th percentiles, the median line within the box and whiskers extending to the minimum and maximum values of the dataset (n = 7/group). *P <0.05, ***P <0.001, ****P <0.0001 relative to 0-hour poststroke baseline scores (n = 7/group); #P <0.05, ##P <0.01, ###P <0.001, ####P <0.0001 relative to pretransplant scores (Kruskal–Wallis ANOVA followed by Dunn’s test). Effect of transplanting vehicle, undifferentiated hNSCs, or predifferentiated cells on infarct area (c), (d) and total infarct volume (e) within the cortex and striatum. Data presented as mean ± SEM of infarct area measured at eight predetermined coronal planes through the brain (two-way ANOVA followed by Bonferroni post test)