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. 2015 May 27;4(11):e1023973. doi: 10.1080/2162402X.2015.1023973

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© 2015 Taylor & Francis Group, LLC

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Figure 1. — Generation of CBDCs from CB-derived CD34⁺ stem cells. (A) Different protocols for generating DCs from CB-derived CD34⁺ cells. (B) Expansion of CD34⁺ cells for the different protocols. (C–E) Phenotype of DCs for the G4, GFS4 and GFS15 protocols. (C) Gating strategy with flow cytometry and cytospins of DCs stained with May–Grünwald–Giemsa. (D) Expression of CD1c, CD1a, CD141, CLEC9A, CD207, CD209, BDCA2, CD123, CD14, CD16, CD64 and CD56 on DCs compared to isotype control. (E) Number of DCs generated from CD34⁺ cells. Data represent at least three independent experiments.