FIG. 6.

Levels of type I IFNs in PBMC stimulated with poly (I:C). Bioassay for type I IFNs was used to measure content of total type I IFN (a) in supernatants from PBMC stimulated with poly (I:C) (10 μg/mL) for 24 h. The results for each patient (n=15) and control (n=16) are displayed as means of duplicates. IFN-β (b) content was estimated using a modified bioassay with neutralizing anti-IFN-β antibodies in 7 patients and 5 controls [using the same supernatants as in (a)]. Nonparametric Mann–Whitney U-test was used to test for statistical differences between patients and controls (*P<0.05).