Table 3.
Primer pairs for each target gene for quantitative real-time PCR
| Gene Name | Primer sequence (5′-3′) | Product size | Annealing temperature (°C) |
|---|---|---|---|
| (bp) | |||
| Up-regulated | |||
| AFLA_125090 | AGGTTGTTCTCGGTCTGGTT | 137 | 65 |
| GCAAGGTCACCTACATGCAC | |||
| AFLA_075280 | AGCTGGTTCGGTTTACCATC | 128 | 64.5 |
| ATGGCGATAGGGACAGGTAG | |||
| AFLA_057960 | TGCAGACCAATGTTCATCCT | 108 | 64.5 |
| GTTGTCTCAGTCGTGCCAGT | |||
| AFLA_039390 | CGCAACAAAGCAAGACATTT | 128 | 61.4 |
| GTCGGAGGGCTTGATTGTAT | |||
| AFLA_077590 | CGTCGATTATGATGGAGACG | 65 | 58.9 |
| CACTGCTCAGCATTCCGTAT | |||
| AFLA_021650 | GTTGGGCTATACGGAGGTGT | 137 | 58.9 |
| GCCATAGAGCAGCCAGTACA | |||
| AFLA_075300 | GTGATTCATAGGGCCGACTT | 156 | 64.5 |
| GACGACAAGGTCTCCGGTAT | |||
| AFLA_023780 | CTCCTCGACCGATTACCATT | 125 | 57.1 |
| CCCACTAACCACATCGACAG | |||
| Down-regulated | |||
| AFLA_117290 | TTGCCCTCTGTTGAAGACTG | 92 | 65 |
| CCTCGTAGGACTTCCTCAGC | |||
| AFLA_112010 | CAGTCGAACCCTATCCACCT | 145 | 58.9 |
| TACGTTCGGAGACACGGATA | |||
| AFLA_137320 | AGCTACCCAGCACCAGAAGT | 76 | 58.9 |
| TGTAGGCAGGAGTCTGTTCG | |||
| AFLA_117420 | CGGCAGAAGAGTACTGGTGA | 106 | 57.1 |
| ACGACTGGTGGTGACGATAA | |||
| AFLA_117340 | TACAACCGGCTACAGCTCAC | 113 | 57.1 |
| TGATCGACTCGGAAAGACTG | |||
| AFLA_058990 | ACGAGTCCTACCACCAGTCC | 64 | 55 |
| TTGATGGTTCCTCCTCCTTC | |||
| AFLA_128370 | CCCTCTTTGGTAAGAATCGC | 144 | 55.8 |
| CGTCGGCCATATTCACATAG | |||
| AFLA_129450 | GTACAGCCGTGGGATTCTTT | 112 | 55.8 |
| TGGAGCCAGTAGATATTGCG |