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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Cold Spring Harb Protoc. 2014 Oct 23;2014(12):1249–1258. doi: 10.1101/pdb.prot084582
Reagent Amount for 1mL (μL) Final Concentration
Tris-HCl* (1 M; pH 7.5; see recipe) 25 200 mM
MgCl2* (1 M) 200 200 mM
NaCl* (5 M) 10 50 mM
Spermidine* (1 M) 10 10mM
DEPC-treated H2O (see recipe) to 10 mL