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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Cold Spring Harb Protoc. 2014 Oct 23;2014(12):1249–1258. doi: 10.1101/pdb.prot084582
Reagent Amount for 500 mL (mL) Final Concentration
Tris-HCl (1 M; pH 9.5; see recipe) 50 100 mM
NaCl (5 M) 10 150 mM
MgCl2 (1 M) 2.5 5 mM
H2O to 500 mL –