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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Cold Spring Harb Protoc. 2014 Oct 23;2014(12):1249–1258. doi: 10.1101/pdb.prot084582
Reagent Amount for 50mL (mL) Final Conc
Tris-HCl* (1 M; pH 7.5) 0.5 10 mM
NaCl* (5 M) 1.5 150 mM
EDTA* (0.5 M; pH 8.0) 0.005 0.05 mM
tRNA* (Type X; 20 μg/μL; Invitrogen Cat. No. 1501-011) 0.125 50 ng/μL
Sodium Dodecyl Sulfate* (SDS; 20%) 0.25 0.1% (v/v)
DEPC-treated H2O (see recipe) to 50 –