| Reagent | Amount for 32μL (μL) | Final Concentration |
|
| ||
| Deionized Formamide* | 16 | 50% (v/v) |
| SSPE 20X conc.* (see recipe) | 3.2 | 2X conc |
| tRNA* (Type X; 20 μg/μL; Invitrogen Cat. No. 15401-011) | 3.2 | 2 μg/μL |
| Bovine Serum Albumin* (BSA; 20μg/μL) | 1.6 | 1 μg/μL |
| Polyadenylic acid potassium salt* (20 μg/μL) | 1.6 | 1 μg/μL |
| Purified Riboprobe | 2.0 | 6.25% (v/v) |
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