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. Author manuscript; available in PMC: 2015 Sep 30.
Published in final edited form as: Cell Metab. 2015 Aug 4;22(2):239–252. doi: 10.1016/j.cmet.2015.07.015

Figure 5. Increased DNA Damage Worsened by miR200 Overexpression in Neurons Differentiated from Medalist +C Type 1 Diabetes Patients.

Figure 5

(A) Immunostaining analyses of pH2AX (marker for DNA damage) and Neu-N (marker for neurons) in neuronal-like cells obtained from directed differentiation of human iPSCs from the three clinical groups including Control 2 and Control 3 as healthy controls, M4 and M14 as Medalists without neuropathy, and M6 and M9 as Medalists with neuropathy (pH2AX, green; Neu-N, red; DAPI, blue). Scale bar: 50 µm.

(B) Quantitation of nuclear H2Ax foci across multiple experiments described in (A).

(C) Immunostaining analyses of pH2AX (marker for DNA damage) and Neu-N (marker for neurons) in neuronal-like cells obtained from directed differentiation of human iPSCs from the three clinical groups post-miR200 overexpression (48 hr) (pH2AX, green; Neu-N, red; DAPI, blue).

(D) Quantitation of nuclear H2Ax foci across multiple experiments described in (A). Scale bar: 50 µm.

See also Figure S6. All experiments were repeated three times, represented as mean ± SD. *p value < 0.001.