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. Author manuscript; available in PMC: 2016 Feb 13.
Published in final edited form as: Nature. 2015 Aug 5;524(7564):180–185. doi: 10.1038/nature14863

Extended figure 4. FACS sorting gates for GFP+ cells in Axin2-rtTA;TetO-H2B-GFP mice.

Extended figure 4

8 week old Axin2-rtTA;TetO-H2B-GFP mice were labeled with doxycycline for 7 days and chased for various lengths of time. Hepatocytes were enzymatically dispersed and sorted by FACS. Successive gating shows sequential selection of (a) all hepatocytes, (b) single cells by forward scatter, (c) side scatter. d, Dead cells were excluded by propidium iodide labeling. e, GFP positive cells were gated and either sorted for RNAseq analysis or further graphed as histograms for GFP intensity analysis (see figure 3g).