Fig 3. Using shape parameters to distinguish hermaphrodites, males and larvae.

Images of 131 worms in three classes (46 hermaphrodites, 46 males, 39 larvae) were analyzed for the shape parameters. Raw data are listed in S1 Table. (A) Distribution of shape parameters in each worm class. (B) Phenotypic profiles using mean values of shape parameters. Z-scores were used to construct the heat map, displaying differences between mean values from a given class and hermaphrodites in multiple standard deviations. σ, standard deviation. (C) Using length and R2 to distinguish three different worm classes.