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. Author manuscript; available in PMC: 2015 Sep 30.
Published in final edited form as: Surgery. 2012 May 8;152(5):869–878. doi: 10.1016/j.surg.2012.03.003

Fig 4.

Fig 4

mRNA of SDF-1 and CXCR4 were expressed in LECs and hepatocytes. A, Before and after 3 hours of hypoxic conditions, total RNA was extracted from LECs and hepatocytes. Representative RT-PCR showed that LECs stably expressed SDF-1 mRNA and the expression of CXCR4 mRNA was increased in heptatocytes by hypoxia. B, Western blot analysis showed that treatment of hepatocytes with SDF-1– and LEC-conditioned medium induced ERK phosphorylation in a dose-dependent manner. C, Treatment of hepatocytes with SDF-1– and LEC-conditioned medium prevented cell death induced by hypoxia. Hepatocytes treated with SDF-1– or LEC-conditioned medium were subjected to hypoxia. AMD3100 (0.1 μg/mL) were added to the hepatocytes treated with LEC-conditioned medium 1 hour before hypoxic culture. Cell viability under hypoxia was measured by MTT assay (*P < .05 compared with hepatocytes without stimulation, #P < .05 compared with hepatocytes treated with LEC-conditioned medium, n = 4–7). The number of cells before hypoxic insult was defined as 100%.