Western blot analysis of lysosomal-associated membrane protein type 2A (LAMP2A). (A) Western blots (upper panels) and quantitative analysis (lower panels) of LAMP2A in homogenate (H), P1, P2, and P3 subcellular fractions after traumatic brain injury (TBI). Samples were prepared from the neocortical tissues of sham-operated control rats and rats subjected to TBI followed by 4 h and 1, 3, 5, and 15 days recovery. Data are normalized with β-actin and are expressed as mean±standard deviation (SD) of fold of control (n=4). *p<0.05 between sham and TBI animals, one-way analysis of variance followed by the Tukey post hoc test. (B) Western blots (upper panel) and quantitative analysis (lower panels) of deglycosylated (deglyc.) LAMP2A in P1 fraction. Data are normalized with β-actin and are expressed as mean±SD of fold of control (n=3). *p<0.05 between sham and TBI animals, unpaired t test.