FIG. 3.

Serum selenium parameters in the Scly KO mice fed a high-fat diet for 4 months. (A) Serum levels of selenoprotein P (Sepp1) and glutathione peroxidase 3 (GPx3) selenoproteins, as measured by Western blot with specific antibodies. Protein quantification is displayed in arbitrary units, with intensity of band of interest normalized by band intensity from two different bands of the Ponceau stained membrane used for the Western blot; n = 6 for each group. (B) Total GPx activity in serum of WT and Scly KO mice; n = 6 for each group. (C) Total selenium content measured in serum of mice by inductively coupled plasma–mass spectrometry (ICP-MS); n = 5 per group. Values are mean ± SEM; **p < 0.01; ns, not significant, by two-tailed Student's unpaired t-test with 95% confidence interval. Black bars: WT mice; white bars: Scly KO mice.