Table 2.

Hepatic Gene Expression of Selenoprotein and Metabolic Enzymes in Wild-Type and Selenocysteine Lyase Knockout Mice After High-Fat Diet Exposure, Assessed by Quantitative Polymerase Chain Reaction

Gene	WT	Scly KO	p-Value (S)	p-Value (MW)
Selenoproteins
Gpx1	0.2487 ± 0.05190	0.2844 ± 0.05585	0.6705	0.8571
Gpx4	0.2161 ± 0.04166	0.3184 ± 0.06741	0.2930	0.4000
Sepp1	3.369 ± 0.2381	3.898 ± 0.4684	0.4102	0.4000
Seps1	0.0116 ± 0.00146	0.0099 ± 0.0013	0.4368	0.4000
Sephs2	0.3505 ± 0.05785	0.3222 ± 0.06640	0.7710	1.0000
Txnrd1	0.0266 ± 0.00139	0.0263 ± 0.00198	0.9042	0.8571
Metabolism
Acaca	0.0446 ± 0.00683	0.0455 ± 0.00693	0.9361	0.8571
Acly	0.0683 ± 0.01948	0.0858 ± 0.01733	0.5386	1.0000
Glut4	0.000682 ± 7.96e-005	0.00079 ± 0.00016	0.6237	0.6286
Me1	0.2337 ± 0.02116	0.2198 ± 0.02572	0.7094	1.0000
Pcx	0.0722 ± 0.00241	0.0553 ± 0.00914	0.1853	0.4000
Pdha1	0.0892 ± 0.00752	0.0867 ± 0.00768	0.8344	1.0000
Sephs1	0.0045 ± 0.00068	0.0045 ± 0.0006	0.9438	0.8571

Values are mean ± SEM and were normalized to GAPDH mRNA levels. Two-tailed Student's t-test (S) and nonparametric Mann–Whitney U test (MW) were used to compare averages. p-Values under 0.05 deemed significant; n = 3–4.