. 2015 Oct 1;23(10):761–774. doi: 10.1089/ars.2015.6277

Table 3.

Gene Expression of Selenoproteins and Metabolic Factors in Murine Hepa1-6 Cells After Silencing of Selenocysteine Lyase and Treatment with 0.2 mM Palmitate Conjugated to 0.46% Bovine Serum Albumin

	Control		siScly		2WA
Gene	BSA	BSA+palmitate	BSA	BSA+palmitate	p_interaction	p_silencing	p_palmitate
Selenoproteins
Gpx1	0.6322 ± 0.07965	0.3773 ± 0.0855	0.5282 ± 0.06174	0.2699 ± 0.1231	0.9847	0.259	*0.0118*
Sepp1	5.659 ± 0.6671	4.946 ± 1.244	2.294 ± 0.7195	2.110 ± 1.048	0.7845	*0.0049*	0.6431
Seps1	0.05223 ± 0.01123	0.03507 ± 0.00728	0.06991 ± 0.02474	0.05375 ± 0.01313	0.9749	0.2585	0.299
Sephs2	0.1108 ± 0.01611	0.05521 ± 0.00987	0.09871 ± 0.02973	0.05935 ± 0.01601	0.68	0.8391	*0.026*
Txnrd1	0.7692 ± 0.12	0.843 ± 0.2057	0.5747 ± 0.1234	1.206 ± 0.153	0.0901	0.5935	*0.0365*
Metabolism
Acaca	0.1171 ± 0.01405	0.06887 ± 0.00865	0.1325 ± 0.0288	0.09256 ± 0.011072	0.8140	0.2796	*0.0224*
Acly	0.2047 ± 0.0203	0.1428 ± 0.01343	0.2292 ± 0.0224	0.2013 ± 0.02165	0.4031	0.0518	*0.0374*
Pcx	0.00608 ± 0.00077	0.004037 ± 0.000516	0.005586 ± 0.00115	0.003068 ± 0.00044	0.7611	0.3577	*0.0093*
Pdh	0.4727 ± 0.2312	0.3167 ± 0.0199	0.3865 ± 0.04269	0.3825 ± 0.02862	*0.0217*	0.7364	*0.0165*
Scly	0.04952 ± 0.00909	0.03197 ± 0.004212	0.02167 ± 0.004734	0.01664 ± 0.003269	0.2945	*0.0018*	0.0684
Sephs1	0.07055 ± 0.0124	0.03462 ± 0.006565	0.09806 ± 0.03064	0.05776 ± 0.009865	0.9023	0.1685	*0.0453*

Values are mean ± SEM, and were normalized to Cphn2 levels. Average comparison by two-way ANOVA (2WA) with Bonferroni's post-test. p-Values were achieved by comparison with control, BSA-only parental cells, and values under 0.5 deemed significant and highlighted in bold/italic; n = 5 per group.

ANOVA, analysis of variance; BSA, bovine serum albumin.