Table 4.
Polymerase Chain Reaction Primer Sequences Used in This Study
| Gene | Forward primer | Reverse primer | Amplicon size (bp) | Product Tm (°C) |
|---|---|---|---|---|
| 18S | CGATTGGATGGTTTAGTGAGG | AGTTCGACCGTCTTCTCAGC | 87 | 90 |
| Acaca | AAATGACCCATCTGTAATGC | TCAATCTCAGCATAGCACTG | 105 | 82 |
| Acly | GCAGCAAAGATGTTCAGTAAA | GTCTGGGTTGTTTATCGATTTT | 129 | 82 |
| Cphn2 | GGAGATGGCACAGGAGGAA | GCCCGTAGTGCTTCAGCTT | 76 | 81 |
| GAPDH | TGACATCAAGAAGGTGGTGAA | CCCTGTTGCTGTAGCCGTATTC | 203 | 78.2 |
| Gpx1 | ACAGTCCACCGTGTATGCCTTC | CTCTTCATTCTTGCCATTCTCCTG | 238 | 82.2 |
| Gpx4 | TCTGTGTAAATGGGGACGATGC | TCTCTATCACCTGGGGCTCCTC | 174 | 77.5 |
| Glut4 | CCCCAGATACCTCTACATCA | CATCCTTCAGCTCAGCTAGT | 110 | 86 |
| Hprt1 | TCCTCCTCAGACCGCTTTT | CCTGGTTCATCATCGCTAATC | 90 | 88 |
| Me1 | CAACAAGGACTTGGCTTTTAC | AATTATTCTAAGGACCTGGAGC | 106 | 82 |
| Pcx | GGGCGGAGCTAACATCTA | CTCGAACTGTTTGGAACTTCA | 70 | 79 |
| Pdh | GTAAGAGTGACCCTATTATGT | ACTTCCACATCAATCCTTT | 91 | 76 |
| Scly | TCCACTCTATGGTAAGATGCT | CTGTGCAAGTGATAAAATGG | 108 | 83 |
| Sepp1 | CCTTGGTTTGCCTTACTCCTTCC | TTTGTTGTGGTGTTTGTGGTGG | 199 | 75.7 |
| Seps1 | CAGAAGATTGAAATGTGGGACAGC | CCTTTGGGGATGACAGATGAAGTAG | 116 | 80 |
| Sephs1 | TGAACTGAAAGGCACAGGCTGC | CGCAAGTATCCATCCCAATGC | 143 | 75 |
| Sephs2 | ACCGACTTCTTTTACCCCTTGG | TCACCTTCTCTCGTTCCTTTTCAC | 166 | 75.6 |
| Txnrd1 | CCTATGTCGCCTTGGAATGTGC | ATGGTCTCCTCGCTGTTTGTGG | 244 | 77 |