Fig 9. Inhibition of endocytosis by cytochalasin D attenuates the toxicity of β2-m amyloid fibrils.

HIG-82 cells preincubated with Ham’s F12 medium containing 0 to 1.0 μg/ml cytochalasin D (CytoD) for 2 hrs, were incubated with the medium containing vehicle buffer or 100 μg/ml β2-m fibrils in the presence of CytoD for 2 days. LDH releasing assay (A) and MTT reduction assay (B) were performed as described in Materials and Methods. Data normalized to positive control and vehicle in LDH releasing assay and MTT reduction assay, respectively, were presented as mean ± SD of three independent experiments. Statistical analysis was performed by Student’s unpaired t-test. *P < 0.05, **P < 0.01.